Anti-beta Catenin (phospho Y654)抗体(ab59430)

概述

• 产品名称
  Anti-beta Catenin (phospho Y654)抗体
  参阅全部 beta Catenin 一抗
• 描述
  兔多克隆抗体to beta Catenin (phospho Y654)
• 特异性
  Detects endogenous levels of beta Catenin only when phosphorylated at tyrosine 654
• 经测试应用
  适用于: ELISA, WBmore details
• 种属反应性
  与反应: Human
  预测可用于: Mouse, Rat
• 免疫原

  Synthetic phosphopeptide derived from human beta Catenin around the phosphorylation site of tyrosine 654 (A-T-Y^P-A-A).

• 阳性对照
  • 293 cells

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• 存储溶液
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 纯化说明
  The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Cadherins

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Catenins

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Cell adhesion
  • Cadherins

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Leukocyte recruitment
  • Other

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

应用

靶标

• 功能
  Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
  Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
• 组织特异性
  Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
• 疾病相关
  Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
  Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
  Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
  Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
  Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
• 序列相似性
  Belongs to the beta-catenin family.
  Contains 12 ARM repeats.
• 翻译后修饰
  Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
  EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
  Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
• 细胞定位
  Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.

• Target information above from: UniProt accession P35222 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 1499 Human
  • Entrez Gene: 12387 Mouse
  • Entrez Gene: 84353 Rat
  • Omim: 116806 Human
  • SwissProt: P35222 Human
  • SwissProt: Q02248 Mouse
  • SwissProt: Q9WU82 Rat
  • Unigene: 476018 Human

  • Unigene: 291928 Mouse
  • Unigene: 112601 Rat

  see all
• 别名
  • Beta catenin antibody
  • Beta-catenin antibody
  • Cadherin associated protein antibody

  • Catenin (cadherin associated protein), beta 1, 88kDa antibody
  • Catenin beta 1 antibody
  • Catenin beta-1 antibody
  • CATNB antibody
  • CHBCAT antibody
  • CTNB1_HUMAN antibody
  • CTNNB antibody
  • CTNNB1 antibody
  • DKFZp686D02253 antibody
  • FLJ25606 antibody
  • FLJ37923 antibody
  • OTTHUMP00000162082 antibody
  • OTTHUMP00000165222 antibody
  • OTTHUMP00000165223 antibody
  • OTTHUMP00000209288 antibody
  • OTTHUMP00000209289 antibody

  see all

实验方案

• Western blot protocols