Anti-beta Catenin (phospho Y489)抗体(ab119801)
Key features and details
- Rabbit polyclonal to beta Catenin (phospho Y489)
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-beta Catenin (phospho Y489)抗体
参阅全部 beta Catenin 一抗 -
描述
兔多克隆抗体to beta Catenin (phospho Y489) -
宿主
Rabbit -
特异性
Immunogen has high homology to the conserved site in gamma-Catenin (Y480). -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- A431 cells and cell lysates treated with pervanadate.
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常规说明
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab119801 was cross-adsorbed to phospho-beta Catenin (phospho Y654) peptide before affinity purification using phospho-beta Catenin (Y489) peptide (without carrier). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119801于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 85 kDa).
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ICC |
1/250.
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说明 |
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WB
1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 85 kDa). |
ICC
1/250. |
靶标
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功能
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
组织特异性
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. -
疾病相关
Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. -
序列相似性
Belongs to the beta-catenin family.
Contains 12 ARM repeats. -
翻译后修饰
Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. - Information by UniProt
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数据库链接
- Entrez Gene: 1499 Human
- Entrez Gene: 12387 Mouse
- Entrez Gene: 84353 Rat
- Omim: 116806 Human
- SwissProt: P35222 Human
- SwissProt: Q02248 Mouse
- SwissProt: Q9WU82 Rat
- Unigene: 476018 Human
see all -
别名
- b-catenin antibody
- Beta catenin antibody
- Beta-catenin antibody
see all
图片
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Lanes 1-2 : Anti-gamma Catenin at 1/1000 dilution
Lanes 3-4 : Anti-beta Catenin (phospho Y489) antibody (ab119801) at 1/1000 dilution
Lanes 5-6 : Anti-beta Catenin at 1/1000 dilution
Lanes 1 & 3 & 5 : A431 cell lysate, stimulated with
pervanadate (1 mM) for 30 min
Lane 2 : A431 cell lysate, stimulated with
pervanadate (1 mM) for 30 min then
treated with akaline phosphatase
Lanes 4 & 6 : A431 cell lysate, stimulated with
pervanadate (1 mM) for 30 min then treated with akaline phosphatase
Predicted band size: 85 kDa -
ab119801 at 1/250 dilution staining beta Catenin in A431 cells treated with pervanadate by Immunocytochemistry followed by detection with secondary antibody conjugated to Cy3.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab119801 被引用在 2 文献中.
- Yu L et al. Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway. Bioact Mater 20:598-609 (2023). PubMed: 35846837
- An S et al. Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies. Ocul Surf 17:589-614 (2019). PubMed: 30965123