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Our Abpromise guarantee covers the use of ab11350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 94 kDa. The peptide is highly phosphorylated in the cytosol, and thus is very unstable. When it is translocated to the nucleus, it is at a lower phosphorylation state, and is therefore more stable. You may be seeing degradation products of the protein, since it may be enzymatically degraded in the cytosol. A 50kDa form is present only if the proteosome is inhibited (by MG132 for example)|
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 5 - 10 µg/ml.|
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