概述

  • 产品名称Anti-beta Catenin抗体[BDI770]
    参阅全部 beta Catenin 一抗
  • 描述
    小鼠单克隆抗体[BDI770] to beta Catenin
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 35-50 of beta Catenin (dephosphorylated).

  • 阳性对照
    • Cell lysate of LiCl treated SW480 cells.

性能

应用

Our Abpromise guarantee covers the use of ab19452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
  • 应用说明ELISA: Use at a concentration of 0.05 µg/ml. IP: Use 1-10µg for 106 vanadate treated A431 cells. WB: Use at a concentration of 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.1ug/ml for HRPO/ECL detection . Predicted molecular weight: 86 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
      Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
    • 组织特异性Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
    • 疾病相关Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
      Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
      Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
      Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
      Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
      Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
    • 序列相似性Belongs to the beta-catenin family.
      Contains 12 ARM repeats.
    • 翻译后修饰Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
      EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
      Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
    • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta catenin antibody
      • Beta-catenin antibody
      • Cadherin associated protein antibody
      • Catenin (cadherin associated protein), beta 1, 88kDa antibody
      • Catenin beta 1 antibody
      • Catenin beta-1 antibody
      • CATNB antibody
      • CHBCAT antibody
      • CTNB1_HUMAN antibody
      • CTNNB antibody
      • CTNNB1 antibody
      • DKFZp686D02253 antibody
      • FLJ25606 antibody
      • FLJ37923 antibody
      • OTTHUMP00000162082 antibody
      • OTTHUMP00000165222 antibody
      • OTTHUMP00000165223 antibody
      • OTTHUMP00000209288 antibody
      • OTTHUMP00000209289 antibody
      see all

    Anti-beta Catenin antibody [BDI770] 图像

    • All lanes : Anti-beta Catenin antibody [BDI770] (ab19452) at 0.5 µg/ml

      Lane 1 : Whole cell lysates of serum starved tumor A431 cells
      Lane 2 : Whole cell lysates of serum starved tumor SW480 cells
      Lane 3 : Whole cell lysates of serum starved tumor SW620 cells
      Lane 4 : Whole cell lysates of serum starved tumor HT29 cells
      Lane 5 : Whole cell lysates of serum starved tumor MCF7 cells
      Lane 6 : Whole cell lysates of serum starved tumor MDA-MB231 cells
      Lane 7 : Whole cell lysates of serum starved tumor T47D cells

      Lysates/proteins at 20000 cells per lane.

      Secondary
      A HRP-conjugated antibody
      Developed using the ECL technique

      Predicted band size : 86 kDa


      Exposure time : 30 seconds

    Anti-beta Catenin antibody [BDI770] (ab19452)参考文献

    This product has been referenced in:
    • Edwards CM  et al. Increasing Wnt signaling in the bone marrow microenvironment inhibits the development of myeloma bone disease and reduces tumor burden in bone in vivo. Blood 111:2833-42 (2008). Read more (PubMed: 18094333) »

    See 1 Publication for this product

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