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Recombinant full length protein (Chicken).
Our Abpromise guarantee covers the use of ab6301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||Use at an assay dependent concentration. PubMed: 21205793|
|EMSA||Use at an assay dependent concentration. PubMed: 12533679|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Beta Catenin knockout HAP1 whole cell lysate (0 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab6301 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab6301 was shown to recognize beta Cateninin wild type cells as signal was lost at the expected MW in beta Catenin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and beta Catenin knockout samples were subjected to SDS-PAGE. Ab6301 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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