Anti-beta Catenin抗体[15B8] (ab6301)

概述

  • 产品名称Anti-beta Catenin抗体[15B8]
    参阅全部 beta Catenin 一抗
  • 描述
    小鼠单克隆抗体[15B8] to beta Catenin
  • 特异性Recognises beta-catenin in immunoblotting. Does not recognise plakoglobin.
  • 经测试应用适用于: WB, IHC-P, EMSA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Zebrafish
  • 免疫原

    Recombinant full length protein (Chicken).

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: A431; HEK293; Caco2; NIH3T3; PC12.

性能

应用

Our Abpromise guarantee covers the use of ab6301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 21205793
EMSA Use at an assay dependent concentration. PubMed: 12533679
ICC/IF 1/1000.

靶标

  • 功能Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 组织特异性Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • 疾病相关Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • 序列相似性Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • 翻译后修饰Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Anti-beta Catenin antibody [15B8] 图像

  • All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-beta Catenin antibody [15B8] (ab6301)参考文献

This product has been referenced in:
  • Rodríguez-Fernández L  et al. Isoform-specific function of calpains in cell adhesion disruption: studies in postlactational mammary gland and breast cancer. Biochem J 473:2893-909 (2016). Read more (PubMed: 27402795) »
  • Wang T  et al. Inhibition of transient receptor potential channel 5 reverses 5-Fluorouracil resistance in human colorectal cancer cells. J Biol Chem 290:448-56 (2015). Read more (PubMed: 25404731) »

See all 9 Publications for this product

Product Wall

Thank you for your inquiry and your patience.

Unfortunately, I am sorry to confirm we would not expect ab2365 and ab15180 to detect the Hydra beta catenin protein as the alignment is not expected to be high with the immunogens.

I ...

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Thank you for contacting us. Having checked our catalog for the appropriate products, I would like to send you the following recommendations. Where possible I looked for both monoclonal and polyclonal antibodies, so if not mentioned we unfortunately d...

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The antibodies in question have not had their epitope fully charachterised so I am unable to answer this question and I do not have another product that I can recommend. If you cannot find what you are looking for within the Abcam catalog, I suggest th...

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Unfortunately, this antibody has not been tested in formalin fixed, parafin embedded tissues. However, it is most likely that it would work but we can't say for certain until it has been tested.

We ahve data for cross reactivity to human, dog and bovine but not on mouse. Since it react with these it is likely to cross react across all mammals. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased ...

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This antibody has not been tested against mouse, so its reactivity with that species is unknown.

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