概述

  • 产品名称Anti-beta Casein抗体
    参阅全部 beta Casein 一抗
  • 描述
    兔多克隆抗体to beta Casein
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 32-62 of Human beta Casein (NP_001882.1).

  • 阳性对照
    • MDA-MB435 cell line lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell line: T24/83

性能

应用

Our Abpromise guarantee covers the use of ab112595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 25 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Important role in determination of the surface properties of the casein micelles.
  • 组织特异性Mammary gland specific. Secreted in milk.
  • 序列相似性Belongs to the beta-casein family.
  • 翻译后修饰Form 1-P is phosphorylated once; half of the molecules are phosphorylated on Ser-24, half on Ser-25.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta-casein antibody
    • CASB antibody
    • CASB_HUMAN antibody
    • Casein beta antibody
    • CSN 2 antibody
    • Csn2 antibody
    see all

Anti-beta Casein antibody 图像

  • ICC/IF image of ab112595 stained T24/83 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112595 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-beta Casein antibody (ab112595) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 25 kDa

Anti-beta Casein antibody (ab112595)参考文献

This product has been referenced in:
  • Zonneveld MI  et al. Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures. J Extracell Vesicles 3:N/A (2014). WB ; Human . Read more (PubMed: 25206958) »

See 1 Publication for this product

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