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Our Abpromise guarantee covers the use of ab31294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 46 kDa).Can be blocked with Beta Arrestin 2 peptide (350-360) (ab45728).|
|IP||Use at an assay dependent concentration. PubMed: 21193245|
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab31294 immunoprecipitating Beta Arrestin 2 in mouse heart whole cell lysate. 50µg of cell lysate was incubated with primary antibody (undiluted) and matrix (Protein A/G Magnetic Beads) for 45 minutes. For western blotting a rabbit anti-Beta Arrestin 2 monoclonal antibody and HRP-conjugated anti-rabbit IgG secondary antibody were used to confirm successful immunoprecipation.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"