概述

  • 产品名称
    Anti-beta Amyloid 37-42抗体
  • 描述
    兔多克隆抗体to beta Amyloid 37-42
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to beta Amyloid 37-42 (C terminal).

性能

应用

Our Abpromise guarantee covers the use of ab34376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/3000.
WB 1/400. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).

1/1000 for analysis of the recombinant protein, 1/400 for tissue extracts or cell lysates.

图片

  • Anti-beta Amyloid 37-42 antibody (ab34376) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 87 kDa
    Observed band size: 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    The band observed at 85 kDa could potentially be a cleaved form of beta Amyloid 37-42 due to the presence of a 17 amino acid signal peptide.

实验方案

文献

This product has been referenced in:
  • Lue LF  et al. Amyloid Beta and Tau as Alzheimer's Disease Blood Biomarkers: Promise From New Technologies. Neurol Ther 6:25-36 (2017). Read more (PubMed: 28733956) »
  • Zenaro E  et al. Neutrophils promote Alzheimer's disease-like pathology and cognitive decline via LFA-1 integrin. Nat Med N/A:N/A (2015). Sandwich ELISA ; Mouse . Read more (PubMed: 26214837) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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