概述

  • 产品名称Anti-beta Actin抗体[SP124]
    参阅全部 beta Actin 一抗
  • 描述
    兔单克隆抗体[SP124] to beta Actin
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide near the N-terminus of Human beta Actin.

  • 阳性对照
    • Raji cell lystate, Human Placenta tissue

性能

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应用

Our Abpromise guarantee covers the use of ab115777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200. Predicted molecular weight: 42 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

Anti-beta Actin antibody [SP124] 图像

  • Flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue)

  • Anti-beta Actin antibody [SP124] (ab115777) at 1/200 dilution + Raji cell lystate

    Predicted band size : 42 kDa
  • Staining of Human beta Actin in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115777 at a dilution of 1/200.

Anti-beta Actin antibody [SP124] (ab115777)参考文献

This product has been referenced in:
  • Cai SD  et al. MicroRNA-144 inhibits migration and proliferation in rectal cancer by downregulating ROCK-1. Mol Med Rep 12:7396-402 (2015). Read more (PubMed: 26458302) »
  • Navarro-Tableros V  et al. Recellularization of rat liver scaffolds by human liver stem cells. Tissue Eng Part A 21:1929-39 (2015). IHC-Fr ; Human . Read more (PubMed: 25794768) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"