Anti-beta Actin抗体- Loading Control (ab13822)

概述

  • 产品名称Anti-beta Actin抗体- Loading Control
    参阅全部 beta Actin 一抗
  • 描述
    鸡多克隆抗体to beta Actin - Loading Control
  • 特异性Detects a single clean band at 40kD representing beta actin. Does not cross-react with adult cardiac, smooth or skeletal muscle actin.
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Chinese Hamster
    预测可用于: Rabbit, Chicken, Cow, Dog, Xenopus laevis, Fish
  • 免疫原

    Synthetic peptide derived from residues 1 - 100 of Human beta Actin.

    .

  • 阳性对照
    • This antibody detects beta actin in HeLa cells in WB and IF This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab13822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with Human beta Actin peptide (ab13772).

靶标

Anti-beta Actin antibody - Loading Control 图像

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : CHO-K1 cell lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa


    Exposure time : 90 seconds
  • ICC/IF image of ab13822 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab13822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC image of beta Actin staining in THuman normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. A goat-anti chicken HRP secondary antibody  (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-beta Actin antibody - Loading Control (ab13822)参考文献

This product has been referenced in:
  • Peng X  et al. Cellular plasticity induced by anti-a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies. Ann Neurol 77:381-98 (2015). WB . Read more (PubMed: 25369168) »
  • Wei SC  et al. Matrix stiffness drives epithelial-mesenchymal transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway. Nat Cell Biol 17:678-88 (2015). Human . Read more (PubMed: 25893917) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification Hippocampal homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 13 2014

Application Western blot
Sample Rabbit Cell lysate - whole cell (corneal stromal cells)
Loading amount 10 µg
Specification corneal stromal cells
Gel Running Conditions Reduced Denaturing (4-12% gel)
Blocking step milk diluent (KPL) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Loading amount 10 µg
Specification HUVEC
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step milk diluent (KPL) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Adipocyte)
Loading amount 5 µg
Specification Adipocyte
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human fibroblast)
Loading amount 5 µg
Specification human fibroblast
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Neonatal Cardiomyocytes)
Loading amount 20 µg
Specification Neonatal Cardiomyocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jun 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"