Anti-beta Actin抗体[AC-15] (HRP) (ab49900)

概述

  • 产品名称Anti-beta Actin抗体[AC-15] (HRP)
    参阅全部 beta Actin 一抗
  • 描述
    小鼠单克隆抗体[AC-15] to beta Actin (HRP)
  • 偶联物HRP
  • 特异性This antibody labels specifically ß-actin in a wide variety of tissues and species. It does not stain adult cardiac and skeletal muscles except for contaminants.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Carp, Monkey
    预测可用于: a wide range of other species不与反应: Drosophila melanogaster, Dictyostelium discoideum
  • 免疫原

    Synthetic peptide corresponding to beta Actin (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    DDDIAALVIDNGSGK

  • 阳性对照
    • Chicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts. Rat dorsal root ganglion and Rat liver lysates

性能

应用

Our Abpromise guarantee covers the use of ab49900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/25000 - 1/50000. Predicted molecular weight: 42 kDa.

靶标

Anti-beta Actin antibody [AC-15] (HRP) 图像

  • All lanes : Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/50000 dilution

    Lane 1 : A549 Lysate
    Lane 2 : HeLa Lysate
    Lane 3 : 3T3 Lysate
    Lane 4 : NRK Lysate
    Lane 5 : MDCK Lysate
    Lane 6 : MDBK Lysate
    Lane 7 : BHK Lysate
    Lane 8 : COS7 Lysate
    Lane 9 : CFB Lysate

    Developed using the ECL technique

    Predicted band size : 42 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa

    Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400.

    Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 (ab6672), anti- IL-17 at 1:3000, anti-TGF-β at 1:1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.

    Normalization of results was ensured by running parallel Western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.

  • Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

Anti-beta Actin antibody [AC-15] (HRP) (ab49900)参考文献

This product has been referenced in:
  • Ambade A  et al. Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease. World J Gastroenterol 22:4091-108 (2016). WB ; Mouse . Read more (PubMed: 27122661) »
  • Gruber R  et al. YAP1 and TAZ Control Pancreatic Cancer Initiation in Mice by Direct Up-regulation of JAK-STAT3 Signaling. Gastroenterology 151:526-39 (2016). WB ; Mouse . Read more (PubMed: 27215660) »

See all 45 Publications for this product

Product Wall

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Application Western blot
Sample Rat Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Fat-adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Feb 09 2016

Application Western blot
Sample Human Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Fat-adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Feb 09 2016

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Jan 08 2014

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Application Western blot
Sample Rat Cell lysate - whole cell (ParC5 rat parotid cell line)
Loading amount 50 µg
Specification ParC5 rat parotid cell line
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
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提交于 Sep 28 2010

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Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 10 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Mar 19 2010

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount 30 µg
Specification Cos-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Sep 20 2007

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Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 30 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Sep 20 2007

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Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 30 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Sep 20 2007

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Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 30 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jun 21 2007

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Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 20 µg
Specification 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"