Anti-beta Actin抗体[AC-15] (HRP) (ab49900)

概述

  • 产品名称
    Anti-beta Actin抗体[AC-15] (HRP)
    参阅全部 beta Actin 一抗
  • 描述
    小鼠单克隆抗体[AC-15] to beta Actin (HRP)
  • 偶联物
    HRP
  • 特异性
    This antibody labels specifically ß-actin in a wide variety of tissues and species. It does not stain adult cardiac and skeletal muscles except for contaminants.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Carp, Monkey
    预测可用于: a wide range of other species不与反应: Drosophila melanogaster, Dictyostelium discoideum
  • 免疫原

    Synthetic peptide corresponding to beta Actin (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    DDDIAALVIDNGSGK

  • 阳性对照
    • Chicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts. Rat dorsal root ganglion and Rat liver lysates

性能

应用

Our Abpromise guarantee covers the use of ab49900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/25000 - 1/50000. Predicted molecular weight: 42 kDa.

靶标

图片

  • All lanes : Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/50000 dilution

    Lane 1 : A549 Lysate
    Lane 2 : HeLa Lysate
    Lane 3 : 3T3 Lysate
    Lane 4 : NRK Lysate
    Lane 5 : MDCK Lysate
    Lane 6 : MDBK Lysate
    Lane 7 : BHK Lysate
    Lane 8 : COS7 Lysate
    Lane 9 : CFB Lysate

    Developed using the ECL technique

    Predicted band size : 42 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa

    Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400.

    Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 (ab6672), anti- IL-17 at 1:3000, anti-TGF-β at 1:1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.

    Normalization of results was ensured by running parallel western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.

  • Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

实验方案

文献

This product has been referenced in:
  • Wang H  et al. Role of the orexin (hypocretin) system in contextual fear conditioning in rats. Behav Brain Res 316:47-53 (2017). Read more (PubMed: 27575948) »
  • Xiang Q  et al. Suppression of FOXM1 Transcriptional Activities via a Single-Stranded DNA Aptamer Generated by SELEX. Sci Rep 7:45377 (2017). WB . Read more (PubMed: 28358012) »

See all 85 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Fat-adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Application
Western blot
Sample
Human Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Fat-adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2016

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (ParC5 rat parotid cell line)
Loading amount
50 µg
Specification
ParC5 rat parotid cell line
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
10 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 19 2010

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
30 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
30 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
20 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册