概述

  • 产品名称Anti-beta Actin抗体[8H10D10]
    参阅全部 beta Actin 一抗
  • 描述
    小鼠单克隆抗体[8H10D10] to beta Actin
  • 经测试应用适用于: WB, ICC/IF, Flow Cyt, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

  • 阳性对照
    • NIH/3T3, Jurkat, Hela, CHO, PC12, HEK293, COS, A549 or MCF-7 cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab101173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA 1/10000.

靶标

  • 功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • 序列相似性Belongs to the actin family.
  • 翻译后修饰ISGylated.
  • 细胞定位Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [8H10D10] 图像

  • All lanes : Anti-beta Actin antibody [8H10D10] (ab101173)

    Lane 1 : NIH/3T3
    Lane 2 : Jurkat
    Lane 3 : HeLa
    Lane 4 : CHO
    Lane 5 : PC12
    Lane 6 : HEK293
    Lane 7 : COS
    Lane 8 : A549
    Lane 9 : MCF-7


    Predicted band size : 42 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • ab101173 staining beta Actin in SKBR-3 (left) and A549 (right) cells by Immunocytochemistry/Immunofluorescence. A Cy3-conjugated Goat polyclonal to Mouse IgG was used as the secondary antibody. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.
  • ab101173 staining beta Actin in MCF-7 cells (right) against staining using a negative control (left)

Anti-beta Actin antibody [8H10D10] (ab101173)参考文献

This product has been referenced in:
  • Huang L  et al. Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome. PLoS One 9:e88893 (2014). WB . Read more (PubMed: 24586430) »
  • Zhang SQ  et al. Exome sequencing identifies MVK mutations in disseminated superficial actinic porokeratosis. Nat Genet 44:1156-60 (2012). Read more (PubMed: 22983302) »

See all 2 Publications for this product

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