Application
Western blot
Sample
Sclerotinia sclerotiorum Cell lysate - whole cell (non)
Loading amount
35 µg
Specification
non
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: skim milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit Santa Cruz
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/100000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 13 2011