Application
Western blot
Sample
Human Cell lysate - whole cell (293 human embryonic kidney)
Loading amount
5 µg
Specification
293 human embryonic kidney
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0,5% Tween20
Secondary antibody
Secondary antibody
None used
Detection
Detection method
FLA-9000 Starion
Exposure
1 minute(s) and 45 second(s)
Bands
Specific: 55 kDa
Additional data
Additional Notes
Lysis Buffer was 1% Triton X-100, 20mM TrisHCl pH 7.5, 10mM EGTA, 150mM NaCl, protease inhibitors.
Lane1: 5µg of Hek-cell lysate
Lane2: 40µg of Hek-cell lysate
The image was acquired by scanning the blot with a FLA-9000 Starion laserscanner at 300V and 200µm resolution. The above mentioned exposure time is just the time it took to scan the blot but does not correlate to signal intensity.
Lane1: 5µg of Hek-cell lysate
Lane2: 40µg of Hek-cell lysate
The image was acquired by scanning the blot with a FLA-9000 Starion laserscanner at 300V and 200µm resolution. The above mentioned exposure time is just the time it took to scan the blot but does not correlate to signal intensity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 15 2010