重组HRP Anti-beta Synuclein抗体[EP1537Y] (ab215783)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1537Y] to beta Synuclein
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-beta Synuclein抗体[EP1537Y]
参阅全部 beta Synuclein 一抗 -
描述
HRP兔单克隆抗体[EP1537Y] to beta Synuclein -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Mouse brain, Human brain
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1537Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab215783于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa). |
靶标
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功能
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease. -
组织特异性
Expressed predominantly in brain; concentrated in presynaptic nerve terminals. -
序列相似性
Belongs to the synuclein family. -
翻译后修饰
Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6620 Human
- Entrez Gene: 104069 Mouse
- Omim: 602569 Human
- SwissProt: Q16143 Human
- SwissProt: Q91ZZ3 Mouse
- Unigene: 90297 Human
- Unigene: 200843 Mouse
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别名
- 14 kDa brain-specific protein antibody
- Beta-synuclein antibody
- Phosphoneuroprotein 14 antibody
see all
图片
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All lanes : HRP Anti-beta Synuclein antibody [EP1537Y] (ab215783) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215783 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab215783 尚未被引用在任何文献中。