重组APC Anti-beta III Tubulin抗体[EP1569Y] - Neuronal Marker (ab224977)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EP1569Y] to beta III Tubulin - Neuronal Marker
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
概述
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产品名称
APC Anti-beta III Tubulin抗体[EP1569Y] - Neuronal Marker
参阅全部 beta III Tubulin 一抗 -
描述
APC兔单克隆抗体[EP1569Y] to beta III Tubulin - Neuronal Marker -
宿主
Rabbit -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): U87MG cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1569Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190574)
- Alexa Fluor® 647 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190575)
- Biotin Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab195903)
- Alexa Fluor® 594 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab201740)
- Alexa Fluor® 555 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab202519)
- Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
- FITC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224978)
- PE Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224979)
- Alexa Fluor® 488 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab283728)
- Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab52623)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224977于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
说明 |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
组织特异性
Expression is primarily restricted to central and peripheral nervous system. -
疾病相关
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
序列相似性
Belongs to the tubulin family. -
结构域
The highly acidic C-terminal region may bind cations such as calcium. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 10381 Human
- Entrez Gene: 22152 Mouse
- Entrez Gene: 246118 Rat
- Omim: 602661 Human
- SwissProt: Q13509 Human
- SwissProt: Q9ERD7 Mouse
- SwissProt: Q4QRB4 Rat
- Unigene: 511743 Human
see all -
别名
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
图片
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Overlay histogram showing U87MG cells stained with ab224977 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224977, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab224977 尚未被引用在任何文献中。