Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell in vitro differentiated for)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
1e+006 cells
Specification
mouse neural stem cell in vitro differentiated for
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBS
Dilution
1/3000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL pico
Negative control
Lane1 - proliferating neural stem cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
Positive control
Lanes 2 and 3 - in vitro differentiated neural stem cells in B27 containing media
Additional data
Additional Notes
Lane1, proliferating neural stem cells, Lanes 2 and 3 - in vitro differentiated neural stem cells in B27 containing media, 24 h and 48 h respectively.
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提交于 Apr 30 2015