Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Main Olfactory Epithelium (MOE))
Permeabilization
Yes - TritonX 0.01%
Specification
Main Olfactory Epithelium (MOE)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Signal very clean in MOE, but more background is seen in lung tissue. Suggest blocking in 5% BSA 2 hours and then 10%GS for 1 hour before primary antibody incubation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jennifer Cisson
Verified customer
提交于 May 11 2015