Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Myoblast/Myotubes)
Permeabilization
Yes - 70% Ethanol and 0.3% Triton in PBS
Specification
Myoblast/Myotubes
Blocking step
CAS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent Solution with BSA
Dilution
1/200
Additional data
Additional Notes
Unfortunately, this antibody does not work very well. In fact, the staining showed below was not different from the negative control. This antibody was tested for WB and works very well. However, for IHC and ICC/IF, based on my experience, this is not a good antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 11 2016