Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (H1299)
Total protein in input
1000 µg
Specification
H1299
Immuno-precipitation step
Protein G
Other product details
Concentration
2 µl/mg lysate
Incubation time
4 hour(s) and 0 minute(s) · Diluent: Lysis Buffer
Western Blot antibody
Name
Abcam antibody:(not specified yet)
Dilution
1/1000
Additional data
Additional Notes
lysate was incubated 2 hours with antibody, then 2 more hours with the addition of matrix.
IP
1-4 10ug each input 5-8 beads alone control 9-12 beads + BCOP Ab
IP
1-4 10ug each input 5-8 beads alone control 9-12 beads + BCOP Ab
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提交于 May 27 2011