Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (15 month TASTPM mouse brain)
Specification
15 month TASTPM mouse brain
Fixative
Formaldehyde
Antigen retrieval step
Other
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: vectorlabs anti Mouse IgGs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
These deposits are really "human".
I have previously negatively reviewed this Ab for Formic acid AR on human brain with amyloid plaques: I shall retest.
This Ab can easily be further diluted and overnight-incubated as I think that the immunostaining seen in the image is too strong.
I have previously negatively reviewed this Ab for Formic acid AR on human brain with amyloid plaques: I shall retest.
This Ab can easily be further diluted and overnight-incubated as I think that the immunostaining seen in the image is too strong.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jun 29 2009