Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (5-20)
Sample
Human Cell lysate - whole cell (HepaRG HepG2)
Specification
HepaRG HepG2
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 3% skim milk
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL prime
Bands
Specific: 42 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
PubMed ID
25101946: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
咲織 辻
Verified customer
提交于 Oct 07 2014