Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
25 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
Additional data
Additional Notes
Tissue solubilised with 1% Triton x-100 in PBS with Roche complete protease inhibitors.
PVDF membrane.
Incubation overnight at 4oC essential with antibody in 5% milk/TBST
PVDF membrane.
Incubation overnight at 4oC essential with antibody in 5% milk/TBST
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提交于 Dec 01 2006