Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
Chemiluminescence
Exposure
10 second(s)
Bands
Specific: 43 kDa
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提交于 Dec 20 2017