Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Mouse Cell lysate - whole cell (Mouse brain lysates)
Specification
Mouse brain lysates
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS/Tween, 5% milk
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 42 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Jul 10 2013