Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
10 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/25000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Mar 19 2010