Anti-beta 4 Defensin抗体[L13-10-D1] (ab14419)


  • 产品名称Anti-beta 4 Defensin抗体[L13-10-D1]
    参阅全部 beta 4 Defensin 一抗
  • 描述
    小鼠单克隆抗体[L13-10-D1] to beta 4 Defensin
  • 经测试应用适用于: ELISA, WB, RIA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: ELDRICGYGT ARCRKKCRSQ EYRIGRCPNT YACCLRK), corresponding to amino acids 25-61 of Human beta 4 Defensin (precursor, SwissProt Q8WTQ1).



Our Abpromise guarantee covers the use of ab14419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at an assay dependent dilution.
    IHC-P: Tested in uterus and kidney sections. Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 8 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
    • 组织特异性High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
    • 序列相似性Belongs to the beta-defensin family.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BD 4 antibody
      • BD-4 antibody
      • BD4 antibody
      • beta 104 antibody
      • Beta defensin 4 antibody
      • Beta-defensin 104 antibody
      • Beta-defensin 4 antibody
      • D104A_HUMAN antibody
      • DEFB-4 antibody
      • DEFB104B antibody
      • Defensin antibody
      • Defensin beta 104 antibody
      • Defensin beta 4 precursor antibody
      • hBD 4 antibody
      • hBD-4 antibody
      • hBD4 antibody
      see all

    Anti-beta 4 Defensin antibody [L13-10-D1] 图像

    • ab14419 staining beta 4 Defensin in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/100 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat IgG polyclonal was used as the secondary antibody.

      See Abreview

    Anti-beta 4 Defensin antibody [L13-10-D1] (ab14419)参考文献

    This product has been referenced in:
    • Dréno B  et al. Hidradenitis Suppurativa: The Role of Deficient Cutaneous Innate Immunity. Arch Dermatol : (2011). ICC/IF ; Human . Read more (PubMed: 22004878) »
    • Garreis F  et al. Roles of human beta-defensins in innate immune defense at the ocular surface: arming and alarming corneal and conjunctival epithelial cells. Histochem Cell Biol 134:59-73 (2010). IHC-P ; Human, Mouse . Read more (PubMed: 20526610) »

    See all 2 Publications for this product

    Product Wall

    Has our customer had a chance to test the protein concentrations via Bradford assay?

    I have unfortunately been unable to locate western blot data. Based on the information it may be that a native western blot system is required. It is also po...

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    Thank you for sending along the dot blots and additional information. We really appreciate the extra troubleshootingeffort.

    Here are my thoughts:

    1. In general it looks like the antibodies are able to detect Defensin in the chorioam...

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    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is are having problems with 8 of our products.  Special thanks for collecting the troubl...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Pancreas)
    Specification Pancreas
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH9
    Permeabilization No

    Dr. Ilka Werner-Martini

    Verified customer

    提交于 Oct 12 2010