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Our Abpromise guarantee covers the use of ab70215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 8 kDa.|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for ELISA with Rabbit polyclonal to beta 4 Defensin (Biotin) (ab70216).|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to beta 4 Defensin (Biotin) (ab70216). Capture|
ab70215 staining beta 40Defensin in the A549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence) treated with cigarette smoke extract. Cells were fixed , permeabilized and blocked. Samples were incubated with primary antibody (1/500) for 1 hour. An Alexa Fluor®488-conjugated was used as the secondary antibody and DAPI used to stain the nuclei.
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