Highly pure (>98%) human recombinant full length beta 4 Defensin
Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.5 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
Immunocytochemistry/ Immunofluorescence - Anti-beta 4 Defensin antibody (ab70215)Image from Pierson T et al., Tobacco Induced Diseases. 2013;11:10:. Fig 2.; doi: 10.1186/1617-9625-11-10.Published by BioMed Central
ab70215 staining beta 40Defensin in the A549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence) treated with cigarette smoke extract. Cells were fixed , permeabilized and blocked. Samples were incubated with primary antibody (1/500) for 1 hour. An Alexa Fluor®488-conjugated was used as the secondary antibody and DAPI used to stain the nuclei.
Pierson T et al. Cigarette smoke extract induces differential expression levels of beta-defensin peptides in human alveolar epithelial cells. Tob Induc Dis11:10 (2013).
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