Anti-beta 3 Adrenergic Receptor抗体(ab94506)


  • 产品名称Anti-beta 3 Adrenergic Receptor抗体
    参阅全部 beta 3 Adrenergic Receptor 一抗
  • 描述
    兔多克隆抗体to beta 3 Adrenergic Receptor
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse beta 3 Adrenergic Receptor aa 350 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab108460, ab108460)

  • 阳性对照
    • This antibody gave a positive signal in mouse brown adipose tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Pancreas.



Our Abpromise guarantee covers the use of ab94506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 10 µg/ml.


  • 功能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
  • 组织特异性Expressed mainly in adipose tissues.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADR B3 antibody
    • ADRB 3 antibody
    • ADRB3 antibody
    • ADRB3_HUMAN antibody
    • ADRB3R antibody
    • Adrenergic beta 3 receptor antibody
    • B3AR antibody
    • Beta 3 adrenoceptor antibody
    • Beta 3 adrenoreceptor antibody
    • Beta 3AR antibody
    • Beta-3 adrenergic receptor antibody
    • Beta-3 adrenoceptor antibody
    • Beta-3 adrenoreceptor antibody
    • Beta3AR antibody
    • FLJ99960 antibody
    see all

Anti-beta 3 Adrenergic Receptor antibody 图像

  • Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/ml + Brown Adipose (Mouse) Tissue Lysate at 20 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of beta 3 Adrenergic Receptor staining in Mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-beta 3 Adrenergic Receptor antibody (ab94506)参考文献

This product has been referenced in:
  • Shimizu I  et al. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112 (2014). WB ; Mouse . Read more (PubMed: 24713652) »
  • Wan Z  et al. IL-6 indirectly modulates the induction of glyceroneogenic enzymes in adipose tissue during exercise. PLoS One 7:e41719 (2012). Read more (PubMed: 22844518) »

See all 2 Publications for this product

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ab94506 is not tested in ICC/IF so it is eligible for testing discount. Are you planning using the knockout cells only or would like to try the normal cells also?

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Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (aortic endothelial cells)
Permeabilization Yes - Saponin 0,1%
Specification aortic endothelial cells
Blocking step PBS/Saponin0,1%/BSA5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative PFA 4%

Miss. Virginie Joris

Verified customer

提交于 Jul 06 2015

Expiration date: 19-06-2012

I am very pleased to hear you would like to accept our offer and test ab94506 in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this ...

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