Anti-beta 2 Microglobulin抗体[B2M-01] (ab759)

概述

  • 产品名称Anti-beta 2 Microglobulin抗体[B2M-01]
    参阅全部 beta 2 Microglobulin 一抗
  • 描述
    小鼠单克隆抗体[B2M-01] to beta 2 Microglobulin
  • 特异性This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA.
  • 经测试应用适用于: ELISA, Flow Cyt, IP, RIA, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rabbit, Chicken, Cow, Dog, Pig
  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照
    • U-937 and RAJI cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration. Dissociation constant of the antibody-soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
WB Use a concentration of 2 µg/ml. Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.

靶标

  • 功能Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • 疾病相关Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • 序列相似性Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 翻译后修饰Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • 细胞定位Secreted. Detected in serum and urine.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    see all

Anti-beta 2 Microglobulin antibody [B2M-01] 图像

  • All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml

    Lane 1 : JEG-3 cell lysate
    Lane 2 : A-431 cell lysate
    Lane 3 : U-937 cell lysate
    Lane 4 : Raji cell lysate

    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 13 kDa
    Lysates were separated on 15% SDS–PAGE gel and electrotransferred to a PVDF membrane. The membrane was blocked in PBS/Tween buffer containing 5% non fat dry milk for 2 hours and probed with ab759 at 4°C overnight.

Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)参考文献

This product has been referenced in:
  • Raha-Chowdhury R  et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). Read more (PubMed: 25896789) »
  • Tiwari S  et al. Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking. J Cell Sci 124:1672-80 (2011). Read more (PubMed: 21511727) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.1M PBS with 3% TritonX100
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 14 2014

Thank you for contacting us for technical support regarding ab759. I have contacted the laboratory and was able to find out that indeed non reducing conditions are advised with this antibody. I have added immediately on our datasheet (accessible belo...

Read More

Thank you for your enquiry. This antibody does not cross react with MHC class I heavy chain.

We do not have any problems with this clone. We have 3 units that are in stock currently.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"