Anti-beta 2 Microglobulin抗体(ab87483)

概述

  • 产品名称Anti-beta 2 Microglobulin抗体
    参阅全部 beta 2 Microglobulin 一抗
  • 描述
    兔多克隆抗体to beta 2 Microglobulin
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Recombinant Fragment
    预测可用于: Human, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse beta 2 Microglobulin.

  • 阳性对照
    • This antibody gave a positive signal in Beta 2 Microglobulin Recombinant protein as well as the following Mouse tissue lysates: Spleen; Thymus; Bone Marrow; Lung. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MEF1 IHC-P: FFPE mouse spleen tissue sections

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).

靶标

  • 功能Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • 疾病相关Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • 序列相似性Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 翻译后修饰Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • 细胞定位Secreted. Detected in serum and urine.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    see all

Anti-beta 2 Microglobulin antibody 图像

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded murine pancreatic tissue sections, labelling beta 2 microglobulin antibody with ab87483 at a dilution of 1/80 incubated for 16 hours at 4°C in 5% BSA diluent. Heat mediated antigen retrival was with 10mM sodium citrate at pH 6.0. Blocking was with 5% serum incubated for 30 minutes at 20°C. Secondary was a biotinated goat anti-rabbit polyclonal biotin conjugate at 1/200.

    See Abreview

  • All lanes : Anti-beta 2 Microglobulin antibody (ab87483) at 1 µg

    Lane 1 : Spleen (Mouse) Tissue Lysate
    Lane 2 : Thymus (Mouse) Tissue Lysate
    Lane 3 : Mouse Bone Marrow Tissue Lysate
    Lane 4 : Mouse lung normal tissue lysate - total protein (ab29297)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 13 kDa
    Additional bands at : 285 kDa,52 kDa,92 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • Anti-beta 2 Microglobulin antibody (ab87483) at 1 µg/ml + Beta 2 Microglobulin Recombinant Protein at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ab87483 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87483 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab87483 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87483, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-beta 2 Microglobulin antibody (ab87483)参考文献

ab87483 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH6
Permeabilization No
Specification pancreas
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"