Anti-beta 2 Adrenergic Receptor抗体(ab69598)

概述

  • 产品名称Anti-beta 2 Adrenergic Receptor抗体
    参阅全部 beta 2 Adrenergic Receptor 一抗
  • 描述
    兔多克隆抗体to beta 2 Adrenergic Receptor
  • 特异性Detects endogenous levels of total beta 2 Adrenergic Receptor protein.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human beta 2 Adrenergic Receptor (internal sequence).

  • 阳性对照
    • NIH/3T3 cells, extracts from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab69598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • 翻译后修饰Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Anti-beta 2 Adrenergic Receptor antibody 图像

  • All lanes : Anti-beta 2 Adrenergic Receptor antibody (ab69598) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HeLa cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • NIH/3T3 cells stained with ab69598 at 1/500 dilution, with and without the immunising peptide.

Anti-beta 2 Adrenergic Receptor antibody (ab69598)参考文献

ab69598 has not yet been referenced specifically in any publications.

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