Anti-beta 2 Adrenergic Receptor抗体(ab61778)


  • 产品名称Anti-beta 2 Adrenergic Receptor抗体
    参阅全部 beta 2 Adrenergic Receptor 一抗
  • 描述
    兔多克隆抗体to beta 2 Adrenergic Receptor
  • 特异性ab61778 detects endogenous levels of total beta 2 Adrenergic Receptor protein.
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human beta 2 Adrenergic Receptor around the phosphorylation site of serine 346.

  • 阳性对照
    • Human brain tissue. HeLa cells.
  • 常规说明

    Based on immunogen sequence homology data ab61778 is predicted to react with mouse species however customers has provided us mixed feedback which is why we no longer support its cross reactivity with mouse samples.  



Our Abpromise guarantee covers the use of ab61778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 46 kDa. (see Abreview)
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
Flow Cyt 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • 功能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • 翻译后修饰Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Anti-beta 2 Adrenergic Receptor antibody 图像

  • ab61778, at 1/50 - 1/100 dilution, staining beta 2 Adrenergic Receptor in paraffin-embedded human brain tissue by immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunising peptide.
  • ab61778, at 1/500 - 1/1000 dilution, staining beta 2 Adrenergic Receptor in HeLa cells by immunofluorescence, in the absence (left panel) or presence (right panel) of the immunising peptide.
  • ab61778 staining the beta 2 adrenergic receptor in Human peripheral blood monomuclear cells by Flow Cytometry. Cells was prepared by Ficoll gradient separation in PBS/BSA and was fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS + 0.5% BSA) for 1 hour at 18°C. Ab7007 (1/100) was used as secondary antibody.
    Gating Strategy: Monocytes

    See Abreview

  • All lanes : Anti-beta 2 Adrenergic Receptor antibody (ab61778)

    Lane 1 : NIH-3T3 cell lysate.
    Lane 2 : NIH-3T3 cell lysate with blocking peptide.

    Predicted band size : 46 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

Anti-beta 2 Adrenergic Receptor antibody (ab61778)参考文献

This product has been referenced in:
  • Sloniecka M  et al. Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ. PLoS One 10:e0134157 (2015). IHC-Fr, ICC/IF ; Human . Read more (PubMed: 26214847) »

See 1 Publication for this product

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Regarding ab61778, I have checked our database and have found that this antibody has been validated in many applications including Western blot and so far, we have no reason to believe that it is not specific. Read More

Thank you for your reply.

The immunogen for ab61778 is a synthetic non-phosphopeptide derived from human beta 2 Adrenergic Receptor around the phosphorylation site of serine 346. Therefore based on the information available on http://www.unip...

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Thank you for contacting Abcam.

The only information we have on ab61778 staining patterns in IHC-P are in the attached file. As I am not an expert in the staining pattern of this antibody, I thought it would be more useful for you to be able ...

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Thank you for contacting us. Unfortunately the peptides used as the immunogens for these products are not available in our catalog.

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for contacting us. I hope that the suggestions which were made help you to resolve the issues which you have been experiencing. As requested, I have arranged for you to receive positive control lysates ab71719 and ab29330 free of charge. T...

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Merci pour votre requête.

A notre connaissance, la réactivité contre le chien n'a pas encore été testée avec l'anticorps ab61778. Par contre l'anticorps ab2900 a lui été testé chez...

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