Anti-beta 1 Adrenergic Receptor抗体(ab85037)

概述

  • 产品名称Anti-beta 1 Adrenergic Receptor抗体
    参阅全部 beta 1 Adrenergic Receptor 一抗
  • 描述
    兔多克隆抗体to beta 1 Adrenergic Receptor
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Sheep, Cow, Cat, Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse beta 1 Adrenergic Receptor.

    (Peptide available as ab97424.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Spinal cord tissue lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab85037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

靶标

  • 功能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
  • 结构域The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
  • 翻译后修饰Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
  • 细胞定位Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADR B1 antibody
    • ADRB 1 antibody
    • ADRB1 antibody
    • ADRB1_HUMAN antibody
    • ADRB1R antibody
    • Adrenergic beta 1 receptor antibody
    • Adrenoceptor beta 1 antibody
    • B1AR antibody
    • Beta 1 adrenoceptor antibody
    • Beta 1 adrenoreceptor antibody
    • Beta-1 adrenergic receptor antibody
    • Beta-1 adrenoceptor antibody
    • Beta-1 adrenoreceptor antibody
    • BETA1AR antibody
    • RHR antibody
    see all

Anti-beta 1 Adrenergic Receptor antibody 图像

  • Anti-beta 1 Adrenergic Receptor antibody (ab85037) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab85037 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85037, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-beta 1 Adrenergic Receptor antibody (ab85037)参考文献

This product has been referenced in:
  • Shimizu I  et al. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112 (2014). WB ; Mouse . Read more (PubMed: 24713652) »
  • Sugatani J  et al. Antiobese function of platelet-activating factor: increased adiposity in platelet-activating factor receptor-deficient mice with age. FASEB J 28:440-52 (2014). Read more (PubMed: 24100020) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (primary culture of cortical rat astrocytes)
Permeabilization Yes - 0.1 % Triton X-100, 10 min
Specification primary culture of cortical rat astrocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 23 2016

Thank you for contacting us.

The host for ab40834 is Goat; you would need an anti goat secondary antibody e.g. ab97100

The host for ab85037 is rabbit; you would need anti rabbit antibody e.g. ab6721

Common secondary can not b...

Read More

Thank you very much for your interest our products.

I have to confirm that we do not have any Anti-beta 1 Adrenergic Receptor antibodies that are tested and guaranteed for bovine samples.

But I am happy to also confirm that the immu...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Neonatal cardiomyocytes)
Specification Neonatal cardiomyocytes
Preparation Cell harvesting/tissue preparation method: Scraper
Sample buffer: 0,5 mM EDTA/PBS
Permeabilization No
Gating Strategy Total cardiomyocytes
Username

Abcam user community

Verified customer

提交于 Jul 19 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"