Anti-beta 1 Adrenergic Receptor抗体(ab85037)
Key features and details
- Rabbit polyclonal to beta 1 Adrenergic Receptor
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-beta 1 Adrenergic Receptor抗体
参阅全部 beta 1 Adrenergic Receptor 一抗 -
描述
兔多克隆抗体to beta 1 Adrenergic Receptor -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Sheep, Cow, Cat, Pig -
免疫原
Synthetic peptide corresponding to Mouse beta 1 Adrenergic Receptor aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97424) -
阳性对照
- This antibody gave a positive signal in Mouse Spinal cord tissue lysate. ICC/IF: THP-1 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85037于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
靶标
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功能
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily. -
结构域
The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor. -
翻译后修饰
Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. -
细胞定位
Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. - Information by UniProt
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数据库链接
- Entrez Gene: 493971 Cat
- Entrez Gene: 281604 Cow
- Entrez Gene: 153 Human
- Entrez Gene: 11554 Mouse
- Entrez Gene: 397355 Pig
- Omim: 109630 Human
- SwissProt: Q9TST6 Cat
- SwissProt: Q9TT96 Cow
see all -
别名
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
see all
图片
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ab85037 staining beta 1 Adrenergic Receptor in THP-1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85037 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Anti-beta 1 Adrenergic Receptor antibody (ab85037) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Mouse Beta-1 adrenergic receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab85037 被引用在 4 文献中.
- Yue W et al. The Apoptotic Resistance of BRCA1-Deficient Ovarian Cancer Cells is Mediated by cAMP. Front Cell Dev Biol 10:889656 (2022). PubMed: 35517499
- D'Adamo P et al. Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse. Metabolism 116:154463 (2021). PubMed: 33309713
- Shimizu I et al. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112 (2014). WB ; Mouse . PubMed: 24713652
- Sugatani J et al. Antiobese function of platelet-activating factor: increased adiposity in platelet-activating factor receptor-deficient mice with age. FASEB J 28:440-52 (2014). PubMed: 24100020