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LLYGEFLIFLLCYYIIRFIYRL, corresponding to amino acids 31-52 of Human Bestrophin. The peptide was amidated for conjugation.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab14927 staining HEK-293 cells transfected with human Bestrophin 1 by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% goat serum for 20 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary. Bestrophin transfected in HEK-293 cells is labeled with green fluorescence. Nuclei were counterstained with DAPI (blue).
This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
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