Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Dog, Turkey, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Medaka fish
Synthetic peptide, corresponding to a region within amino acids 543-593 of Human BEGAIN (NP_065887.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab99945 was affinity purified using an epitope specific to BEGAIN immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
Use at 5-15 µg/mg of lysate.
May sustain the structure of the postsynaptic density (PSD).
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - Anti-BEGAIN antibody (ab99945)
All lanes : Anti-BEGAIN antibody (ab99945) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 65 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-BEGAIN antibody (ab99945)
ab99945 at 1 µg/ml detecting BEGAIN in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilized ECL with a 30 second exposure.
For immunoprecipitation, ab99945 was used at at 10 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1: IP using ab99945
Lane 2: IP using control IgG.
has not yet been referenced specifically in any publications.
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