概述

  • 产品名称Anti-Beclin 1抗体
    参阅全部 Beclin 1 一抗
  • 描述
    兔多克隆抗体to Beclin 1
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    17 amino acid synthetic peptide from near the amino terminus of human Beclin 1

  • 阳性对照
    • 293 cell lysate and rat brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab62557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the beclin family.
  • 细胞定位Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Anti-Beclin 1 antibody 图像

  • Lane 1 : Anti-Beclin 1 antibody (ab62557) at 0.5 µg/ml
    Lane 2 : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
    Lane 3 : Anti-Beclin 1 antibody (ab62557) at 2 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab62557 staining Beclin 1 in Human MCR5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 21°C. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab62557 staining Beclin 1 in Human umbilical vascular endothelial cells after 2 Gy of ionizing radiationby at 4 and 8 days post-irradiation by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% formaldehyde/PBS pH 7.4 for 20 minutes at 37°C, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% PBS with BSA pH 7.4. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. An undiluted Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

  • ab62557 at 2µg/ml staining Beclin 1 in rat brain by Immunohistochemistry.

Anti-Beclin 1 antibody (ab62557)参考文献

This product has been referenced in:
  • Zhao M  et al. Methylene blue exerts a neuroprotective effect against traumatic brain injury by promoting autophagy and inhibiting microglial activation. Mol Med Rep 13:13-20 (2016). IHC ; Mouse . Read more (PubMed: 26572258) »
  • Tan S  et al. Enhancement of oxaliplatin-induced cell apoptosis and tumor suppression by 3-methyladenine in colon cancer. Oncol Lett 9:2056-2062 (2015). WB ; Mouse . Read more (PubMed: 26137012) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 100 µg
Treatment Perifosine
Specification Epithelial OVCA cell line
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 18 2016

Application Western blot
Sample Human Cell lysate - whole cell (H1299)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 60 µg
Treatment different concentrations of a drug
Specification H1299
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Jan 08 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (GONAD)
Antigen retrieval step Enzymatic
Permeabilization No
Specification GONAD
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 22 2015

Application Western blot
Loading amount 75 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Rat Cell lysate - whole cell (gonad)
Specification gonad
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (MRC5)
Specification MRC5
Permeabilization Yes - /0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Nov 13 2013

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