Anti-Beclin 1抗体[2A4] (ab114071)

概述

  • 产品名称Anti-Beclin 1抗体[2A4]
    参阅全部 Beclin 1 一抗
  • 描述
    小鼠单克隆抗体[2A4] to Beclin 1
  • 经测试应用适用于: WB, ELISA, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of human Beclin 1 expressed in E. Coli.

  • 阳性对照
    • WB: Hela; A431; MCF7; RAJI; Jurkat and SKBR3 cell lysates IHC-P: Breast cancer and liver cancer tissues

性能

应用

Our Abpromise guarantee covers the use of ab114071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 60 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the beclin family.
  • 细胞定位Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Anti-Beclin 1 antibody [2A4] 图像

  • All lanes : Anti-Beclin 1 antibody [2A4] (ab114071) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Raji cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : SKBR3 cell lysate


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of Beclin 1 expression in paraffin embedded breast cancer tissues (left) and liver cancer tissues (right) using ab114071 at 1/200 dilution with DAB staining
  • Flow cytometric analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).

Anti-Beclin 1 antibody [2A4] (ab114071)参考文献

This product has been referenced in:
  • Wang J Beclin 1 bridges autophagy, apoptosis and differentiation. Autophagy 4:947-8 (2008). Read more (PubMed: 18769161) »
  • Pickford F  et al. The autophagy-related protein beclin 1 shows reduced expression in early Alzheimer disease and regulates amyloid beta accumulation in mice. J Clin Invest 118:2190-9 (2008). Read more (PubMed: 18497889) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Canine osteosarcoma & mammary tumour)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 15 µg
Treatment 1-2 (OS1) = N/A, 3 (D17) = 75 uM HCQ, 4 (D17) = 75 uM HCQ + doxorubicin, 5 (D17) = 75 uM HCQ + 5 uM Spautin-1, 6 (D17) = 75 uM HCQ + 5 uM Spautin-1 + doxorubicin, 7 (CF41) = N/A, 8 (MCF7) = N/A, 9 (D17) = N/A, 10 (D17) = doxorubicin, 11 (D17) = 15 uM Spautin-1 + doxorubicin, 12 (D17) = 120 uM Spautin-1 + doxorubicin, 13 (SKBR3) = N/A, 14 (OS2) = E64d + pepstatin-A (All treatments 6 hr except lane 14 which was 24 hr)
Specification Canine osteosarcoma & mammary tumour
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Courtney Schott

Verified customer

提交于 Sep 26 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% troton x-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 14 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Permeabilization Yes - 0.3% Triton x 100: 30 min
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 02 2016

Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12)
Loading amount 30 µg
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Md. Golam Sharoar

Verified customer

提交于 Feb 16 2016

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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My colleague John has forwarded you the Flow Cytometry protocol used by our lab, please let us know if you have any further questions about this.

Unfortunately, the concentration for this antibody was no...

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Thank you for contacting Abcam yesterday.

Please find attached the protocol for Flow Cytometry that was used to test ab114071. The secondary antibody used was a AlexaFluor@488goatanti-mouse(IgG(H+C), dilution:1:150.
Please let me know if...

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Je suis ravi que vous souhaitiez participer à notre offre et tester ab114071 en ICC/IF. Le code de réduction correspondant est ******. Ce code de réduction est utilisable sous 120 jours...

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I apologize, I had sent the wrong link.  Here are the two references for ab114071: http://www.abcam.com/Beclin-1-antibody-2A4-ab114071-references.html

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"