概述

  • 产品名称Anti-BDP / ARID3B抗体
    参阅全部 BDP / ARID3B 一抗
  • 描述
    兔多克隆抗体to BDP / ARID3B
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 100 and 150 of Human BDP/ ARID3B (EAW99328.1).

  • 阳性对照
    • 293T whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab92328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
  • 组织特异性Expressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level).
  • 序列相似性Contains 1 ARID domain.
    Contains 1 REKLES domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARI3B_HUMAN antibody
    • ARID domain containing protein 3B antibody
    • ARID domain-containing protein 3B antibody
    • Arid3b antibody
    • AT rich interactive domain 3B BRIGHT like antibody
    • AT rich interactive domain containing protein 3B antibody
    • AT-rich interactive domain-containing protein 3B antibody
    • Bright and dead ringer gene product homologous protein Bdp antibody
    • Bright and dead ringer protein antibody
    • Bright like antibody
    • Bright-like protein antibody
    • Brightlike antibody
    • DRIL2 antibody
    see all

Anti-BDP / ARID3B antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BDP / ARID3B with ab92328 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BDP / ARID3B antibody (ab92328) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 61 kDa


    Exposure time : 3 minutes
  • Detection of BDP / ARID3B by Western Blot of Immunprecipitate.
    ab92328, at 1 µg/ml, staining BDP / ARID3B in 293T whole cell lysate immunoprecipitated using ab92328 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-BDP / ARID3B antibody (ab92328)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - nuclear (J1 embryonic stem cells)
Loading amount 10 µg
Specification J1 embryonic stem cells
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 4°C
Username

Dr. Francesco Faiola

Verified customer

提交于 Apr 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"