概述

  • 产品名称Anti-BDP / ARID3B抗体
    参阅全部 BDP / ARID3B 一抗
  • 描述
    兔多克隆抗体to BDP / ARID3B
  • 特异性Block in 3% milk
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse BDP/ ARID3B.

    (Peptide available as ab30607.)

  • 阳性对照
    • F9 Mouse Embryonic Carcinoma, E14tG2a (Mouse embryonic stem cell line), PC12 (Rat adrenal pheochromocytoma cell line)Whole Cell Lysate; Mouse Thymus, Testis, Thyroid Gland and Cerebellum Tissue Lysate

性能

应用

Our Abpromise guarantee covers the use of ab32481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
  • 组织特异性Expressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level).
  • 序列相似性Contains 1 ARID domain.
    Contains 1 REKLES domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARI3B_HUMAN antibody
    • ARID domain containing protein 3B antibody
    • ARID domain-containing protein 3B antibody
    • Arid3b antibody
    • AT rich interactive domain 3B BRIGHT like antibody
    • AT rich interactive domain containing protein 3B antibody
    • AT-rich interactive domain-containing protein 3B antibody
    • Bright and dead ringer gene product homologous protein Bdp antibody
    • Bright and dead ringer protein antibody
    • Bright like antibody
    • Bright-like protein antibody
    • Brightlike antibody
    • DRIL2 antibody
    see all

Anti-BDP / ARID3B antibody 图像

  • ICC/IF image of ab32481 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32481, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-BDP / ARID3B antibody (ab32481) at 1 µg/ml

    Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : Thyroid Gland (Mouse) Tissue Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate
    Lane 4 : Thymus (Mouse) Tissue Lysate
    Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : Cerebellum (Mouse) Tissue Lysate
    Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 61,70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes
  • IHC image of ab32481 staining in mouse testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32481, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-BDP / ARID3B antibody (ab32481)参考文献

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