Anti-BDNF抗体[EPR1292] (ab108319)


  • 产品名称Anti-BDNF抗体[EPR1292]
    参阅全部 BDNF 一抗
  • 描述
    兔单克隆抗体[EPR1292] to BDNF
  • 特异性This product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human BDNF aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P23560
    (Peptide available as ab182199)

  • 阳性对照
    • WB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate, brain tissue lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells
  • 常规说明

    Our internal data indicates that mouse and rat are not recommended for IHC.

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.Can be blocked with BDNF peptide (ab182199).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/750.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • 组织特异性Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • 疾病相关Bulimia nervosa 2
      Congenital central hypoventilation syndrome
    • 序列相似性Belongs to the NGF-beta family.
    • 翻译后修饰The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Abrineurin antibody
      • ANON2 antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • BULN2 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Anti-BDNF antibody [EPR1292] 图像

    • All lanes : Anti-BDNF antibody [EPR1292] (ab108319) at 1/1000 dilution

      Lane 1 : Human hippocampus lysate
      Lane 2 : Rat hippocampus lysate
      Lane 3 : Mouse hippocampus lysate

      Lysates/proteins at 20 µg per lane.

      Gt anti Rb IR680 at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 15 kDa
      Additional bands at : 15 kDa (possible mature (processed) protein),28 kDa (possible multimer),35 kDa,45 kDa (possible immature (unprocessed)). We are unsure as to the identity of these extra bands.

      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108319 (1/1000) overnight at 4°C. Ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

    • Immunohistochemical analysis of brain tissue from sedentary (SED) or exercised rats (EX), staining BDNF with ab108319.

      Antigen retrieval was performed by enzymatic method at room temperature. Tissue was permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum. Samples were incubated with primary antibody (1/100) for 3 days at 3°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100
    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with ab108319 at a dilution of 1/750. Cells were fixed with Paraformaldehyde and permeabilised with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

      The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

      See Abreview

    Anti-BDNF antibody [EPR1292] (ab108319)参考文献

    This product has been referenced in:
    • Monnier A  et al. Brain-derived neurotrophic factor of the cerebral microvasculature: a forgotten and nitric oxide-dependent contributor of brain-derived neurotrophic factor in the brain. Acta Physiol (Oxf) N/A:N/A (2016). Rat . Read more (PubMed: 27364224) »
    • Liu T  et al. Cannabinoid-Elicited Conditioned Place Preference in a Modified Behavioral Paradigm. Biol Pharm Bull 39:747-53 (2016). WB ; Rat . Read more (PubMed: 26935020) »

    See all 15 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Cell lysate - whole cell (brain)
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Loading amount 15 µg
    Specification brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

    Abcam user community

    Verified customer

    提交于 Apr 21 2017

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (cerebellum)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
    Permeabilization No
    Specification cerebellum
    Fixative formalin

    Dr. Emmanuel Bouchaert

    Verified customer

    提交于 Oct 06 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Sep 29 2016

    Thank you for contacting us.
    We have quite a few antibodies for BDNF. The antibodies ab108319, ab108383 ab109049 or ab72439 are against mature BDNF however ab78367 would detect the pro form.
    I hope this information is helpful to you. Please d...

    Read More

    Thank you for contacting us and for your interest in our products. I think ab108319 (www.abcam.com/ab108319) or ab108383 (www.abcam.com/ab108383) would be suitable since you are looking for a monoclonal antibody. However, please note that none of t...

    Read More