概述

  • 产品名称Anti-BDKRB1抗体
    参阅全部 BDKRB1 一抗
  • 描述
    兔多克隆抗体to BDKRB1
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the internal region of human BDKRB1

  • 阳性对照
    • Extracts from K562 cells. LOVO cells.

性能

应用

Our Abpromise guarantee covers the use of ab75148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B1 bradykinin receptor antibody
    • B1BKR antibody
    • B1R antibody
    • BDKR B1 antibody
    • BDKRB 1 antibody
    • BDKRB1 antibody
    • BK 1 receptor antibody
    • BK-1 receptor antibody
    • BKB1R antibody
    • BKR 1 antibody
    • BKR1 antibody
    • BKRB1_HUMAN antibody
    • BRADY B1 antibody
    • BRADYB1 antibody
    • Bradykinin B1 receptor antibody
    • Bradykinin receptor 1 antibody
    • Bradykinin receptor B1 antibody
    see all

Anti-BDKRB1 antibody 图像

  • All lanes : Anti-BDKRB1 antibody (ab75148) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 48 kDa,50 kDa,82 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of BDKRB1 in LOVO cells using ab75148, at 1/500 dilution, with (right panel) or without (left panel) preincubation with the immunizing peptide.

Anti-BDKRB1 antibody (ab75148)参考文献

This product has been referenced in:
  • Ghebrehiwet B  et al. Soluble gC1qR is an autocrine signal that induces B1R expression on endothelial cells. J Immunol 192:377-84 (2014). WB . Read more (PubMed: 24319267) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Healthy and CLL B-cells, SW837 (rectum adenocarcin)
Loading amount 40 µg
Specification Healthy and CLL B-cells, SW837 (rectum adenocarcin
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Elena Kashuba

Verified customer

提交于 Mar 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"