重组
RabMAb

Anti-BCRP/ABCG2抗体[EPR2099(2)] (ab108312)

概述

  • 产品名称
    Anti-BCRP/ABCG2抗体[EPR2099(2)]
    参阅全部 BCRP/ABCG2 一抗
  • 描述
    兔单克隆抗体[EPR2099(2)] to BCRP/ABCG2
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the intracellular domain of Human BCRP/ABCG2.

  • 阳性对照
    • Human fetal liver, JAR, 293T, HepG2, and Human placenta lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能
      Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
    • 组织特异性
      Highly expressed in placenta. Low expression in small intestine, liver and colon.
    • 序列相似性
      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • 翻译后修饰
      Glycosylation-deficient ABCG2 is normally expressed and functional.
    • 细胞定位
      Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ABC transporter antibody
      • ABC15 antibody
      • ABCG 2 antibody
      • ABCG2 antibody
      • ABCG2_HUMAN antibody
      • ABCP antibody
      • ATP binding cassette sub family G (WHITE) member 2 antibody
      • ATP binding cassette transporter G2 antibody
      • ATP-binding cassette sub-family G member 2 antibody
      • BCRP antibody
      • BCRP1 antibody
      • BMDP antibody
      • Breast cancer resistance protein antibody
      • CD338 antibody
      • CDw338 antibody
      • CDw338 antigen antibody
      • EST157481 antibody
      • GOUT1 antibody
      • MGC102821 antibody
      • Mitoxantrone resistance associated protein antibody
      • Mitoxantrone resistance-associated protein antibody
      • MRX antibody
      • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
      • MXR antibody
      • MXR1 antibody
      • Placenta specific ATP binding cassette transporter antibody
      • Placenta specific MDR protein antibody
      • Placenta-specific ATP-binding cassette transporter antibody
      • UAQTL1 antibody
      see all

    图片

    • All lanes : Anti-BCRP/ABCG2 antibody [EPR2099(2)] (ab108312) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : JAR lysate
      Lane 3 : 293T lysate
      Lane 4 : HepG2 lysate
      Lane 5 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa

    实验方案

    文献

    This product has been referenced in:
    • Pan X  et al. Downregulation of eIF4G by microRNA-503 enhances drug sensitivity of MCF-7/ADR cells through suppressing the expression of ABC transport proteins. Oncol Lett 13:4785-4793 (2017). WB . Read more (PubMed: 28599480) »
    • Zhan Y  et al. Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy. Oncol Lett 12:2511-2516 (2016). Read more (PubMed: 27698820) »

    See all 3 Publications for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (human liver)
    Permeabilization
    No
    Specification
    human liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    methanol:acetone (1:1 ratio)
    Username

    Abcam user community

    Verified customer

    提交于 Oct 30 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (ES-derived hepatocytes)
    Permeabilization
    Yes - BSA diluted in PBS/0.1% Tween
    Specification
    ES-derived hepatocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    提交于 Dec 12 2013

    Thank you for contacting us.

    For ab108312, you could use human fetal liver (ab29890), JAR, 293T (ab95494), HepG2 (ab7900) or human placenta (ab29745) lysates as a positive control.

    For ab32574, you could consider using MRP-1 trans...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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