Anti-BCRP/ABCG2抗体[BXP-53] (ab24115)

概述

  • 产品名称
    Anti-BCRP/ABCG2抗体[BXP-53]
    参阅全部 BCRP/ABCG2 一抗
  • 描述
    大鼠单克隆抗体[BXP-53] to BCRP/ABCG2
  • 宿主
    Rat
  • 经测试应用
    适用于: ICC, WB, Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: EMSA
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Fusion protein containing the E. coli maltose binding protein and a fragment corresponding to amino acids 221-394 of Mouse BCRP/ABCG2

  • 表位
    Reacts with an internal epitope of BCRP/ABCG2.

性能

应用

Our Abpromise guarantee covers the use of ab24115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/20 - 1/50. Acetone fixed cytospin.
WB 1/20 - 1/50. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
Flow Cyt Use 1-2µg for 106 cells.

(1/10 dilution on bone marrow and kidney tissue).

 

 

 

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration. PubMed: 20472681
ICC/IF Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for EMSA.
  • 靶标

    • 功能
      Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
    • 组织特异性
      Highly expressed in placenta. Low expression in small intestine, liver and colon.
    • 序列相似性
      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • 翻译后修饰
      Glycosylation-deficient ABCG2 is normally expressed and functional.
    • 细胞定位
      Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ABC transporter antibody
      • ABC15 antibody
      • ABCG 2 antibody
      • ABCG2 antibody
      • ABCG2_HUMAN antibody
      • ABCP antibody
      • ATP binding cassette sub family G (WHITE) member 2 antibody
      • ATP binding cassette transporter G2 antibody
      • ATP-binding cassette sub-family G member 2 antibody
      • BCRP antibody
      • BCRP1 antibody
      • BMDP antibody
      • Breast cancer resistance protein antibody
      • CD338 antibody
      • CDw338 antibody
      • CDw338 antigen antibody
      • EST157481 antibody
      • GOUT1 antibody
      • MGC102821 antibody
      • Mitoxantrone resistance associated protein antibody
      • Mitoxantrone resistance-associated protein antibody
      • MRX antibody
      • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
      • MXR antibody
      • MXR1 antibody
      • Placenta specific ATP binding cassette transporter antibody
      • Placenta specific MDR protein antibody
      • Placenta-specific ATP-binding cassette transporter antibody
      • UAQTL1 antibody
      see all

    图片

    • All lanes : Anti-BCRP/ABCG2 antibody [BXP-53] (ab24115) at 1 µg/ml

      Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 72 kDa
      Observed band size: 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      BCRP/ABCG2 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Overlay histogram showing HEK293 cells stained with ab24115 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24115, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • ab24115 staining BCRP/ABCG2 in murine mesenchymal stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.1% Triton X and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/25 for 15 hours at 25°C. The secondary antibody used was a donkey anti-rat IgG conjugated to an Alexa Fluor® used at a 1/100 dilution.

      See Abreview

    文献

    This product has been referenced in:
    • Wang W  et al. SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition. J Exp Clin Cancer Res 37:37 (2018). WB ; Human . Read more (PubMed: 29475441) »
    • Zhang W  et al. Rab23 promotes the cisplatin resistance of ovarian cancer via the Shh-Gli-ABCG2 signaling pathway. Oncol Lett 15:5155-5160 (2018). Read more (PubMed: 29552151) »

    See all 24 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (BRAIN)
    Antigen retrieval step
    Other
    Permeabilization
    Yes - TRITON 1%
    Specification
    BRAIN
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Mrs. Francoise Geffroy

    Verified customer

    提交于 Jun 20 2018

    Free of charge replacement being sent.

    Thank you for taking the time to contact us and provide the details. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case f...

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    Thank you for your reply.

    I have to confirm that we did not test this antibody in IF with paraffin embedded sections.

    This is in theory possible. Although often high background occurs with this method.

    Please follow this l...

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    Thank you for your inquiry.

    I am happy to confirm that both antibodies are tested and guarantee for IHC-P.

    1.) We used ab52816 successfully in a dilution of 1/100 in IHC-P with human squamous cervical carcinoma by Immunohistochemist...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab24115.

    To check the status of the order please contact o...

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    Thank you for your reply. As we had previously discussed, ab24115 is not in an ideal formulation for use with our conjugation kits due to the fact that is not a purified antibody and it contains BSA as a stabilizer protein. I am sorry that these produc...

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    Thank you for contacting us.


    I have discussed the EMSA results with my colleagues, and to be honest we do not have any specificsuggestions:

    Because there are many unique requirements for each nucleic acid binding protein and th...

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    Thank you for contacting Abcam. Unfortunately we do not have a blocking peptide available for this product. The sequence that was used to generate this antibody was ******** of Mouse BCRP/ABCG2 (SwissProt: Q7TMS5). You could have a peptide synthesized ...

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    DISCOUNT CODE: xxx Expiration date:dd mm 2012  I am very pleased to hear you would like to accept our offer and test ab24115 in EMSA. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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