概述

  • 产品名称Anti-Bcr抗体
    参阅全部 Bcr 一抗
  • 描述
    兔多克隆抗体to Bcr
  • 特异性ab51087 is capable of detecting endogenous levels of total Bcr protein.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Non-phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N)

  • 阳性对照
    • K562 cell extracts.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 159 kDa (predicted molecular weight: 143 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
  • 疾病相关Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • 序列相似性Contains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • 结构域The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
    The DH domain is involved in interaction with CCPG1.
  • 翻译后修饰Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALL antibody
    • bcr antibody
    • BCR/ABL FUSION GENE, INCLUDED antibody
    • BCR/FGFR1 chimera protein antibody
    • BCR/FGFR1 FUSION GENE, INCLUDED antibody
    • BCR/PDGFRA FUSION GENE, INCLUDED antibody
    • BCR_HUMAN antibody
    • BCR1 antibody
    • Breakpoint cluster region antibody
    • Breakpoint cluster region protein antibody
    • CML antibody
    • D22S11 antibody
    • D22S662 antibody
    • FGFR1/BCR chimera protein antibody
    • PHL antibody
    • Renal carcinoma antigen NY-REN-26 antibody
    see all

Anti-Bcr antibody 图像

  • All lanes : Anti-Bcr antibody (ab51087) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : K562 cell extract + blocking peptide


    Predicted band size : 143 kDa
    Observed band size : 159 kDa (why is the actual band size different from the predicted?)
  • Ab51087 staining human colon. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Bcr antibody (ab51087)参考文献

ab51087 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (K562 Cell Lysate)
Specification K562 Cell Lysate
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Type Sandwich (Detection)
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提交于 Dec 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"