重组Anti-Bcr-abl抗体[7C6] (ab187831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [7C6] to Bcr-abl
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Bcr-abl抗体[7C6]
参阅全部 Bcr-abl 一抗 -
描述
小鼠单克隆抗体[7C6] to Bcr-abl -
宿主
Mouse -
特异性
This antibody recognizes an epitope within the amino acid sequence SSINEEITPRRQS of Bcr/Abl.
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经测试应用
适用于: ICC/IF, WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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表位
Cells from immunized Balb/c mice were fused with the P3X63Ag8 myeloma cell line. -
阳性对照
- WB: K562 whole cell lysate. ICC/IF: K562 cells.
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常规说明
This product has switched from a hybridoma to recombinant production method on 08th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
7C6 -
骨髓瘤
P3-x63-Ag8 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab187831于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/250.
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WB |
1/1000. Predicted molecular weight: 197 kDa.
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说明 |
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ICC/IF
1/250. |
WB
1/1000. Predicted molecular weight: 197 kDa. |
靶标
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相关性
The BCR gene is involved in the 9:22 translocation that generates the Philadelphia chromosome both in chronic myeloid leukemia (CML) and in a proportion of cases of acute lymphocytic leukemia (ALL). A 5' bcr sequence becomes fused to an abl sequence (including tyrosine kinase domain sequences) from chromosome 9 and results in the production of a chimaeric BCR-ABL protein with enhanced kinase activity. These antibodies may be useful for studies of the oncogene and have potential diagnostic and prognostic applications. -
数据库链接
- Omim: 151410 Human
- SwissProt: A9UF07 Human
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别名
- ALL antibody
- BCR ABL fusion antibody
- BCR ABL fusion protein antibody
see all
图片
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 fixed K562 (human chronic myelogenous leukemia lymphoblast) cells labeling Bcr-abl with ab187831 at 1/250 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing strongly cytoplasmic staining in K-562 cell line. The nuclear counter stain id DAPI (Blue). Tubulin is detected using ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red).
The negative controls are as follows:
-ve control 1: ab187831 at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) secondary at 1/500 dilution.
-ve control 2: Primary (ab179513) at 1/200 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1/1000 dilution. -
All lanes : Anti-Bcr-abl antibody [7C6] (ab187831) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 197 kDa
Observed band size: 142,210 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates should be non-boiled as boiling may cause protein aggregates.
Exposure time: 59 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab187831 被引用在 8 文献中.
- Hrdinova T et al. Exosomes released by imatinib-resistant K562 cells contain specific membrane markers, IFITM3, CD146 and CD36 and increase the survival of imatinib-sensitive cells in the presence of imatinib. Int J Oncol 58:238-250 (2021). PubMed: 33491750
- Zhang C et al. KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia. Theranostics 11:2691-2705 (2021). PubMed: 33456567
- Li L et al. Decitabine Downregulates TIGAR to Induce Apoptosis and Autophagy in Myeloid Leukemia Cells. Oxid Med Cell Longev 2021:8877460 (2021). PubMed: 33532040
- Labib M et al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng 5:41-52 (2021). PubMed: 32719513
- Zhang C et al. Growth of tyrosine kinase inhibitor-resistant Philadelphia-positive acute lymphoblastic leukemia: Role of bone marrow stromal cells. Oncol Lett 13:2059-2070 (2017). WB . PubMed: 28454362
- Dhut S et al. BCR-ABL and BCR proteins: biochemical characterization and localization. Leukemia 4:745-50 (1990). PubMed: 2232885
- Dhut S et al. Identification of two normal bcr gene products in the cytoplasm. Oncogene 3:561-6 (1988). PubMed: 3078961
- Walker LC et al. Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia. Nature 329:851-3 (1987). PubMed: 3478595