Anti-BCMA抗体[Vicky-1] (ab17323)


  • 产品名称
    参阅全部 BCMA 一抗
  • 描述
    大鼠单克隆抗体[Vicky-1] to BCMA
  • 宿主
  • 特异性
    Recognizes human BCMA.
  • 经测试应用
    适用于: ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant human BCMA:Fc. The extracellular domain of human BCMA (amino acids 2-54) is fused to the Fc portion of human IgG1.

  • 阳性对照
    • U266 cells



Our Abpromise guarantee covers the use of ab17323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明
    Flow Cyt: Use at a concentration of 1 µg/ml (see recommended protocol and figures).
    ICC: Use at a concentration of 5 µg/ml.
    Functional Application (blocks binding of BAFF to human BMCA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
    • 组织特异性
      Expressed in mature B-cells, but not in T-cells or monocytes.
    • 疾病相关
      Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
    • 序列相似性
      Contains 1 TNFR-Cys repeat.
    • 细胞定位
      Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B cell maturation antigen antibody
      • B cell maturation factor antibody
      • B cell maturation protein antibody
      • B-cell maturation protein antibody
      • BCM antibody
      • BCMA antibody
      • CD269 antibody
      • CD269 antigen antibody
      • TNFRSF17 antibody
      • TNR17_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 17 antibody
      see all


    • Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel).
      Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5µg/ml ab17323 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

    • FACS analysis of cells with ab17323 to BCMA.

      Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of ab17323 monoclonal antibody to human BCMA. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry.

    • Detection of endogenous human BCMA with ab17323 to BCMA (human)(Vicky-1) (second peak, to the right). Method: U266 cells (2x105) were incubated on ice for 30 minutes with 0.2 µg of ab17323 or an isotype control in 25µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide). The primary antibody was revealed with anti- Rat IgG (R-PE) and then analyzed by Flow Cytometry.


    ab17323 has not yet been referenced specifically in any publications.


    Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab17323 has never been tested with samples from cynomolgus monkey. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these a...

    Read More

    I'm sorry to hear the customer is experiencing problems in FACS with some of our antibodies, it is likely that the problem is protocol related. I have received this morning a detailed FACS protocol with details of the buffers to use and fixative to use...

    Read More

    The antibody to BCMA (Vicky-1) has been tested by FACS analysis on several cells (B cells from Myeloma U266 or in B cell Lymphoma suDHL-6). The endogenous protein is well detected. If you have any additional questions, please contact us again.