概述

  • 产品名称Anti-BCL9抗体
    参阅全部 BCL9 一抗
  • 描述
    小鼠单克隆抗体to BCL9
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1036-1136 of Human BCL9 with tag (NP_004317).

  • 阳性对照
    • Recombinant fragment of human BCL9; HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab54833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged BCL9 is approximately 0.03ng/ml if used as a capture antibody.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 149 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use a concentration of 10 µg/ml.

靶标

Anti-BCL9 antibody 图像

  • ab54833 at 10µg/ml staining BCL9 in HeLa cells by Immunofluorescence.
  • Anti-BCL9 antibody (ab54833) at 1/500 dilution + Recombinant fragment of human BCL9 at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 149 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-BCL9 antibody (ab54833)参考文献

This product has been referenced in:
  • Jia W  et al. MicroRNA-30c-2* Expressed in Ovarian Cancer Cells Suppresses Growth Factor-Induced Cellular Proliferation and Downregulates the Oncogene BCL9. Mol Cancer Res 9:1732-45 (2011). WB ; Human . Read more (PubMed: 22024689) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Ameloblasts section)
Permeabilization Yes - 0.5% Tween
Specification Ameloblasts section
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 25°C
Fixative Paraformaldehyde
Username

Claudio Cantù

Verified customer

提交于 Oct 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"