Anti-BCL9抗体(ab37305)
Key features and details
- Rabbit polyclonal to BCL9
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BCL9抗体 -
描述
兔多克隆抗体to BCL9 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human BCL9 aa 800-900 conjugated to keyhole limpet haemocyanin.
(Peptide available asab37304) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37305于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 140 kDa).Can be blocked with Human BCL9 peptide (ab37304).
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 140 kDa).Can be blocked with Human BCL9 peptide (ab37304). |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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相关性
BCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies (referenced from swissprot and entrez gene). -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 607 Human
- Entrez Gene: 77578 Mouse
- Omim: 602597 Human
- SwissProt: O00512 Human
- SwissProt: Q1JQ81 Human
- SwissProt: Q5T489 Human
- SwissProt: Q9D219 Mouse
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别名
- B-cell CLL/lymphoma 9 antibody
- BCL 9 antibody
- Legless homolog antibody
- LGS antibody
图片
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ab37305 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37305 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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All lanes : Anti-BCL9 antibody (ab37305) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab37305 被引用在 20 文献中.
- Zhang X et al. miRNA-122-5p in POI ovarian-derived exosomes promotes granulosa cell apoptosis by regulating BCL9. Cancer Med 11:2414-2426 (2022). PubMed: 35229987
- Wang Z et al. Discovery of an Orally Bioavailable Small-Molecule Inhibitor for the β-Catenin/B-Cell Lymphoma 9 Protein-Protein Interaction. J Med Chem 64:12109-12131 (2021). PubMed: 34382808
- Zimmerli D et al. TBX3 acts as tissue-specific component of the Wnt/ß-catenin transcriptional complex. Elife 9:N/A (2020). PubMed: 32808927
- Salazar VS et al. Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche. Elife 8:N/A (2019). PubMed: 30735122
- Wang L et al. Mechanism of Low Expression of miR-30a-5p on Epithelial-Mesenchymal Transition and Metastasis in Ovarian Cancer. DNA Cell Biol 38:341-351 (2019). PubMed: 30839226